Osteo Science Foundation is proud to award research grants supporting residents and fellows in their effort to bring about new developments and treatment concepts more quickly at the clinical level. We are proud to be their partners and to help lead the way by funding initiatives that are making a true difference.
The following are grant recipients of the Resident Research Award.
CD90+ adipose-derived mesenchymal stem cells in an alveolar extraction socket model
Seth R. Brooks obtained his DDS from the University of Oklahoma College of Dentistry in 2014. Following graduation he completed a 1 year fellowship in Dental Anesthesia at the Indiana University School of Medicine prior to starting his residency in Oral and Maxillofacial Surgery at the University of Tennessee Medical Center in Knoxville.
The proposed study aims to evaluate the use of adult adipose-derived mesenchymal stem cells in bony tissue regeneration of extraction sockets in rats. Due to time, economical and restorative concerns associated with implant placement after dental extractions, bone regenerative therapies have garnered much attention in recent years. Current treatments, based on autologous and allogenic bone grafts, suffer from inherent challenges and hence, the ideal bone replacement therapy is yet to be found. In this proposal, a team of clinicians and researchers at UTMCK along with colleagues at UTCVM will test, in vitro, three scaffolds and determine which is the most biocompatible with adult mesenchymal stem cells. Subsequently we will test the in vivo efficiency of these scaffolds seeded with adult mesenchymal stem cells in a rat model of extraction socket defect. The long term goal is to translate this rat model to a large animal and ultimately make this strategy available to human medicine.
Third Molar Autotransplantation in the Pediatric Patient: Pilot Study
Alex Musser is a Chief Oral and Maxillofacial Surgery resident at the University of Cincinnati. He obtained his dental degree at the University of Louisville.
Abstract: It is well documented in the literature that the autotransplantation of teeth is a clinically successful procedure given the right indications. Currently, the literature is lacking in studies specifically addressing immature third molar to first molar autotransplantation. Without transplant, the patient would need mechanical space maintenance after loss of the first molar. Although mechanical space maintenance is an option, these prosthetics are not esthetic, they do not preserve hard and soft tissue, they do not provide proprioception, and orthodontic treatment is most assuredly needed in the future. By providing esthetics, bone and soft tissue preservation, proprioception and reducing the need for possible future orthodontic treatment, autotransplantation is clearly advantageous to the patient long term. The goals of this study are to evaluate if this surgery is a successful option for replacement of a first molar, occlusal space and alveolar bone maintenance and to provide the background for future studies. Our central hypothesis is that autotransplantation of an immature third molar to a first molar recipient site (using a specifically defined pre-operative, surgical, and post-operative protocol with a multidisciplinary approach) will be a successful alternative to extraction with or without other forms of space maintenance.
Application of Dental Pulp Stem Cells (DPSCs) in Facial Nerve Regeneration
Dr. Pasha Shakoori is a PGY-3 resident in Oral and Maxillofacial Surgery at the Hospital of the University of Pennsylvania and the Children’s Hospital of Philadelphia. He completed dental school and a master’s degree at Columbia University in the City of New York with a focus on stem cell research and biomaterials. His research is in regeneration and applications of regenerative medicine in reconstructive surgery. He will be continuing his research while pursuing a Doctor of Science degree in biomaterials at the University of Pennsylvania while in residency. He is also active in professional organizations where he serves as an executive board member of the Resident Organization of the American Association of Oral and Maxillofacial Surgeons (ROAAOMS).
Dental pulp-derived stem cells (DPSCs) are capable of differentiating into different lineages of neural cells, thus rendering them a promising candidate seed cells for peripheral nerve regeneration. Tissue-engineered nerve conduits with DPSCs have been shown to promote facial nerve regeneration in rats. Our preliminary data showed that DPSCs can be differentiated into both Schwann and neuron-like cells when cultured under 2D- and small-intestine submucosa (SIS) membranes. Based on these findings, we hypothesize that DPSCs seeded on SIS-scaffolds could represent a promising alternative stem cell-based nerve wrap for facial nerve repair/regeneration. Our project aims to optimize the conditions to differentiate DPSCs into both Schwann and neuronal cells under 2D- and SIS 3D-culture conditions. Human DPSCs can be an excellent candidate for peripheral nerve regeneration due to its neural crest origin, sufficient availability, ready accessibility, non-invasive harvesting procedures, rapid proliferation, multipotent differentiation, and successful integration into host tissues with immunologic tolerance. In addition, in combination with tissue engineering technologies, these DPSCs can also serve as a superior seed cell source for the development of engineered nerve products that hold great promises for clinical application for peripheral nerve repair/regeneration.